RESEARCH

• High-yield expression of full-length and partial sequences of the receptor molecules
• Synthesis of novel ligands for and generation of specific antibodies against the two receptors
• Immunological characterization and localization of the receptors in tissues and cultured cells
• Biochemical and physico-chemical characterization of receptor proteins
• Cloning and characterization of angiotensin II receptor-binding proteins

back to top

---

SECTION CHIEF

Hao-Chia Chen received his Ph.D. in Biochemistry from Emory University, Atlanta, Georgia in 1964. He was an Assistant Professor of Biochemistry at the Rockefeller University, New York, before joining NICHD as a Senior Investigator in 1972. He served as Chief, Section on Molecular Structure and Protein Chemistry, from 1983-93 and currently is Head of the Unit of Molecular Structure and Protein Chemistry, ERRB.

Contact Information

Hao-Chia Chen, Ph.D
Endocrinology and Reproduction Research Branch
NICHD, Building 49, Room 6A36
Bethesda, MD 20892-4510
USA

Telephone: 301-496-2861
Fax: 301-402-2403
Email: chen@helix.nih.gov

back to top

---

PERSONNEL

Personnel:

· Hung-Dar Chen, Ph.D., Bldg. 49; Rm. 6C-64; tel: 301-496-6152; e-mail: hdchen@helix.nih.gov
· Wei Li, Ph.D., Bldg. 49; Rm. 6C-64; tel: 301-496-6152; e-mail: liw@mail.nih.gov
· John L. Morell, Chemist, Bldg. 49; Rm. 6B-60; tel: 301-496-6151; e-mail: morell@mail.nih.gov

back to top

---

BIBLIOGRAPHY

Suzuki N, Khoo KH, Chen HC, Johnson JR and Lee YC. (2001) Isolation and Characterization of Major Glycoproteins of Pigeon Egg White. J. Biol. Chem. 276, 23221-23229.

Zhang M, Zhao X, Chen HC, Catt KJ and Hunyady L. (2000) Activation of AT1 Receptor Is Dependent on Adjacent Apolar Residues in the Carboxyl Terminus of the Third Cytoplasmic Loop. J. Biol. Chem. 275, 15782-15788.

Chen HD, Huang, YK, Ault K, Wong GW, Lin MC, Chen HC and Kung HF. (2000) Molecular Basis for Differing Anti-neurogenic Effects of GATA-1a and GATA-1b in Xenopus. Biochem. Biophys. Res. Commun. 273, 614-620.

Huang KP, Huang FL and Chen HC. (1999) Hypoxia/Ischemia Induces Dephosphorylation of Rat Brain Neuromodulin/GAP43 In Vivo. J. Neurochem. 72, 1294-1306.

Lee-Huang S, Huang PL, Sun Y, Huang PL, Kung HF, Blithe DL and Chen HC. (1999) Lysozyme and Ribonucleases as Anti-HIV Components in -Core Preparations of hCG. Proc. Nat'l Acad. Sci. U.S.A. 96, 2678-2681.

Smith RD, Baukal AJ, Zolyomi A, Gaborik Z, Hunyady L, Sun L, Zhang M. Chen HC and Catt KJ. (1998) Phosphorylation of the Endogeneous AT1 Angiotensin Receptor in Bovine Adrenal Glomerulosa Cells: Analysis with a Novel Anti-AT1 Receptor Antibody. Mol. Endocrinol. 12, 634-644.

Cawley NX, Olsen V, Zhang CF, Chen HC, Tan M and Loh YP. (1998) Activation and Processing of Non-anchored Yapsin 1 (Yap3p). J. Biol. Chem. 273, 584-591.

Cool DR, Normant E, Shen FS, Chen HC, Pannell L, Zhang Y and Loh YP. (1997) Carboxypeptidase E is a Sorting Receptor for Regulated Secretory Pathway Proteins and Genetic Obliteration Leads to Endocinological Disorders in the Cpefat Mouse. Cell 88, 73-83.

Cawley NX, Chen, HC, Beinfeld MC and Loh YP. (1996) Specificity and Kinetics Studies on the Cleavage of Various Prohormone Mono- and Paired Basic Residue Sites by Yeast Aspartic Proteases 3. J. Biol. Chem. 271, 4168-4176.

Lee-Huang S, Huang PL, Huang PL, Bourinbaiar AS, Chen HC, and Kung HF. (1995) Inhibition of Human Immunodeficiency Virus Type 1 (HIV-1) Integrase by Anti-HIV Plant Proteins MAP30 and GAP31. Prod. Nat'l Acad Sci. U.S.A. 92, 8818-8822.

Seki K, Chen HC, Huang KP. (1995) Dephosphorylation of Protein Kinase C Substrates, Neurogranin, Neuromodulin and MARCKS, by Calcineurin. Arch. Biochem. Biophys. 316, 673-679.

Lee-Huang S, Kung HF, Huang PaulL, Bourinbaiar AS, Morell JL, Brown JH, Huang PhL, Tsai WP, Chen AY, Huang HI, Chen HC, (1994) HIV-1 inhibition, DNA binding, RNA binding and ribosome inactivation activities in the N-terminal seqments of the plant anti-HIV protein GAP 31. Proc Natl Acad Sci USA 91, 12208-12212.

Hope, J.N., Chen, H.C. and Hejtmancik, J.F. (1994) Aggregation of A3-crystalline is Independent of the Specific Sequence of the Domain Connecting Peptide. J. Biol. Chem. 269, 21141-21145.

Lee-Huang, S., Kung, S.F, Chen, H.C., Huang, P.L., Ryback, S.M., Huang, P.L., Bourinbaiar, A.S., Musayev, F. and Liaw, Y.C. (1994) Crystallization and Preliminary X-ray Analysis of GAP 31: A Protein Which Inhibits the Life Cycle of HIV-1. J. Mol. Biol. 240, 92-94.

Hirata, Y., Kiuchi, K., Chen, H.C., Milbrands, J. and Guroff, G. (1993) The Phosphorylation and DNA Binding of the DNA-Binding Domain of the Orphan Nuclear Receptor NGF1-B. J. Biol. Chem. 268, 24808-24812.

Driscoll, W.J., Martin, B.M., Chen, H.C. and Strott, C.A. (1993) Isolation of Two Distinct 3-Hydroxysteroid Sulfotransferases from the Guinea Pig Adrenal: Evidence for 3 -Hydroxy Versus 3ß-Hydroxy Stereospecificity. J. Biol. Chem. 268, 23496-23503.

Chen, H.C., Morell, J.L. and Brown, J.H. (1993) Structure-Activity Studies of Antibiotic Peptides from Frog Skin. Peptide Chemistry 1992 (Yanaihara, N., Ed.) pp293-296, ESCOM, Publisher, The Netherlands.

Azaryan, A.V., Wong, M., Friedman, T.C., Cawley, N.X., Estivariz, F.E., Chen, H.C. and Loh, Y.P.
(1993) Purification and Characterization of a Paired Basic Residue-Specific Yeast Aspartic Protease Encoded by the YAP3 Gene: Similarity to the Mammalian Pro-opiomelanocortin Converting Enzyme. J. Biol. Chem. 268, 11968-11975.

Lee-Huang, S., Chen, H.C., Kung, H.F., Huang, P.L., Nara, P.L., Li, B.Q., Huang, P., Huang, H.I., and Huang, P.L. (1992) Plant Proteins with Activity against Human Immunodeficiency Virus. in Natural Products as Antiviral Agents (Chu, C.K., and Cutler, H.G., Eds.), pp153-172, Plenum Press, Publisher, New York.

back to top

---